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Analysis of saponins
from black bean by electrospray ionization and fast atom bombardment tandem mass
spectrometry.
J Mass Spectrom 1999 Aug;34(8):804-12
Lee MR, Chen CM, Hwang BH, Hsu LM.
Department of Chemistry, National Chung-Hsing University, Taichung, Taiwan.
Saponins from black bean (Vigna mungo L. Hepper) were analyzed using positive
and negative ion fast atom bombardment mass spectrometry (FAB-MS) and liquid
chromatography/mass spectrometry. Methanol was used to extract the saponins from
defatted black bean, which was partially purified by extraction with n-butanol,
and the extract was dialyzed with 3000 M(r) cut-off tubing. The dialyzate was
analyzed using mass spectrometry. According to FAB-MS/MS, mixtures from black
bean contain soyasaponin I as the predominant saponin. In addition, MS/MS
analysis was performed in which the structures of saponins of black bean
cotyledon were determined to be soyasaponin I, soyasaponin II, soyasaponin V,
3-O-[alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-galactopyranosyl-(1 -->
2)-beta-D-glucuronopyranosyl]complogenin (saponin A) and
3-O-[alpha-L-rhamnopyranosyl-(1 --> 2)-beta-D-glucopyranosyl-(1 -->
2)-beta-D-glucopyranosyl]oleanolic acid (saponin B). For the black bean shell
and the root of black bean sprout, analysis confirmed the saponins of
soyasaponin I, soyasaponin II, soyasaponin V, saponin A, saponin B,
acetylsoyasaponin A(4) and soyasaponin beta(g). Moreover, all the studied
saponins were found in the stem and leaves of the black bean sprouts, except
soyasaponin beta(g) and acetylsoyasaponin A(4), respectively. Copyright 1999
John Wiley & Sons, Ltd.
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