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Biokinetic
studies in humans with stable isotopes as tracers. Part 1: A methodology for
incorporation of trace metals into vegetables.
Isotopes Environ Health Stud 1998;34(3):291-6
Giussani A, Heinrichs U, Roth P, Werner E, Schramel P, Wendler I.
GSF, National Research Center for Environment and Health, Institute of Radiation
Protection, Neuherberg, Germany. augusto.giussani@mi.infn.it
The metabolism and biokinetics of trace metals in humans can be successfully
studied employing stable isotopes of the investigated elements as tracers. For
the estimation of the bioavailability and the intestinal absorption from solid
food, materials are required which have been intrinsically labelled with the
chosen stable tracer, since the use of an extrinsic label may lead to erroneous
results. Here a technique for producing intrinsically labelled vegetables is
presented and optimized with regard to molybdenum, gadolinium and ruthenium,
elements of interest in the field of radiation protection and/or nutrition.
These feasibility studies were aimed to determine the most favourable conditions
for the production of vegetables containing the selected tracers in amounts high
enough to enable successful biokinetic studies in humans. In this optimization
study the natural elements were used instead of the more expensive stable
isotopes. Mo is readily absorbed both into cress (Lepidium sativum) and into
french beans (Phaseolus vulg. var. nanus). Gd uptake into cress is moderate,
while Ru may be easily and successfully incorporated only into sprouts of mung
beans (Vigna radiata).
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