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Change
in the reaction of the antioxidant system of wheat sprouts after UV-irradiation
of seeds
Biofizika. 2000 Jul-Aug;45(4):730-6.
[Article in Russian]
Rogozhin VV, Kuriliuk TT, Filippova NP.
Yakutsk State Agricultural Academy, Russia.
The response of the antioxidant system of sprouts of wheat Triticum aestivum L.
to preliminary irradiation of seeds with UV light was studied. The dependence of
lipid peroxidation and the extent of antioxidant activity on the duration of
irradiation was studied. It was shown that low doses of UV radiation (5-15 min)
stimulate the antioxidant protection of green wheat sprouts grown for eight
days. Increasing the irradiation time to 30-60 min leads to the inhibition of
lipid peroxidation by the antioxidant system. A more prolonged irradiation of
seeds with UV light (for 1-6 h) led to an increase in the level of lipid
peroxidation in sprouts. However, 1-2-day-old sprouts from seeds irradiated for
5-6 h, adapted themselves to the influence due to the compensatory mechanisms.
By the 8th day of germination of preliminarily irradiated seeds, the content of
antioxidants and malone dialdehyde returned to the norm. The dynamics of
activity of peroxidase in seeds irradiated with low doses of UV light for 30 min
was studied. It was found that on the third day of seed germination, a decrease
in peroxidase activity followed by its slight increase occurred. The maximum
activity of the enzyme in the endosperm was observed on day 5-6, and in roots
and green sprouts, on day 3-5 of germination. It was concluded that antioxidants
and peroxidase are involved in the compensatory mechanisms of inhibition of free
radicals formed upon UV irradiation of seeds.
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