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The Effect of Cruciferous and
Leguminous Sprouts on Genotoxicity, in Vitro and in Vivo.
Cancer Epidemiol
Biomarkers Prev. 2004 Jul;13(7):1199-205.
Gill CI, Haldar S, Porter S,
Matthews S, Sullivan S, Coulter J, McGlynn H, Rowland I.
Northern Ireland Centre for Food and Health, University of Ulster, Coleraine,
United Kingdom. C.Gill@ULST.AC.UK
Vegetable consumption is associated with a reduced risk of colorectal cancer,
which is the second most common cancer after lung/breast cancer within Europe.
Some putative protective phytochemicals are found in higher amounts in young
sprouts than in mature plants.The effect of an extract of mixed cruciferous and
legume sprouts on DNA damage induced by H(2)O(2) was measured in HT29 cells
using single cell microgelelectrophoresis (comet). Significant antigenotoxic
effect (P < or = 0.05) was observed when HT29 cells were pre-incubated with the
extract (100 and 200 microL/mL) for 24 hours and then challenged with H(2)O(2).
A parallel design intervention study was carried out on 10 male and 10 female
healthy adult volunteers (mean age = 25.5 years) fed 113 g of cruciferous and
legume sprouts daily for 14 days. The effect of the supplementation was measured
on a range of parameters, including DNA damage in lymphocytes (comet), the
activity of various detoxifying enzymes (glutathione S-transferase, glutathione
peroxidase, and superoxide dismutase), antioxidant status using the ferric
reducing ability of plasma assay, plasma antioxidants (uric acid, ascorbic acid,
and alpha-tocopherol), blood lipids, plasma levels of lutein, and lycopene. A
significant antigenotoxic effect against H(2)O(2)-induced DNA damage was shown
in peripheral blood lymphocytes of volunteers who consumed the supplemented diet
when compared with the control diet (P = 0.04). No significant induction of
detoxifying enzymes was observed during the study, neither were plasma
antioxidant levels or activity altered. The results support the theory that
consumption of cruciferous vegetables is linked to a reduced risk of cancer via
decreased damage to DNA.
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