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Effect of
germination on the protein content and on the level of specific activity of
lipoxygenase-1 in seedlings of three soybean cultivars.
Arch Latinoam Nutr 1995 Sep;45(3):222-6
Bordingnon JR, Ida EL, Oliveira MC, Mandarino JM.
EMBRAPA-CNPSo, Londrina, PR, Brazil.
The effect of germination on the protein content and in the level of specific
activity of lipoxygenase-1 on seedlings of Santa Rosa, FT-2 and Davis soybean
cultivars were studied. Soybean seeds of the three cultivars were germinated
from 0 to 72 hours in a seed germinator at 30 degrees C temperature, 100%
relative humidity, and absence of light. The seeds were then frozen in liquid
nitrogen, freeze-dried and milled. The lipoxygenase-1 was extracted with 0.02M
thris-HCI buffer at pH = 8.2 and 13% saccharose for 30 minutes at 4 degrees C
without shaking. The highest levels of specific activity of lipoxygenase-1 per
milligram of flour (dry basis) in the Santa Rosa, FT-2 and Davis cultivars were
observed between 18 and 36, 18 and 24, and 0 and 12 hours, respectively. The
polinomial quadratic model best fits the regression analysis of the variable
levels of specific lipoxygenase activity for cultivar Davis (R2 = 0.9197).
Although for cultivars Santa Rosa (R2 = 0.9041)., and FT-2 (R2 = 0.7486) the 4th
degree polynomial model has shown the best fit. The protein content in the three
cultivars studied reached the maximum values at 48h germination. The germination
induced a relative increase of the protein content but caused a reduction of the
level of specific lipoxygenase-1 activity.
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