Isoenzyme composition
and some properties of pea lipoxygenase
Biokhimiia 1977 Nov;42(11):2079-85
[Article in Russian]
Borisova IG, Chepurenko NV, Budnitskaia EV.
The isoenzyme composition and some properties of lipoxygenase isolated from the
seeds and 10-day old sprouts of pea plant were studied. The enzyme activity
assay, using gel- and ion-exchange chromatography, disc-electrophoresis in
polyacrylamide gel, etc. revealed that the plant contains two lipoxygenase
systems of unsaturated long-chain fatty acids oxidation. The existence of four
lipoxygenase isoenzymes whose combination determines the type of lipoxygenase-catalyzed
reactions of linoleic acid oxidation, has been confirmed.